Hong Ge, Xiaoqi Gong and Careen K. Tang. "High Incidence of Co-expression of EGFRwt and EGFRvIII Transcripts in Human Invasive Breast Cancer Tissue By Laser Capture Microdissection/RT-PCR." International Journal of Cancer 98.3 (2002): 357-361.
Detection of the presence of EGFRvIII mRNA and revealed a high incidence of EGFRvIII transcript in human primary invasive breast cancer by utilizing laser capture microdissection (LCM)/RT-PCR. In addition, 57.1% of the infiltrating breast carcinomas expressed both EGFRwt and EGFRvIII mRNA in the same tumor. There is no detectable EGFRvIII mRNA in normal breast tissue. Evaluation of the EGFRwt and EGFRvIII protein levels in the same sample sets by immunohistochemical analysis further confirmed the LCM/RT-PCR finding. Our study provides first direct evidence of high incidence of coexpression of EGFRvIII and EGFRwt in human invasive breast cancer tissue. The unique characteristics and high prevalence of EGFRvIII in invasive human breast cancer as well as negative expression in normal breast may suggest its important role in breast carcinogenesis and make it an ideally potential target for treatment of breast cancer without interrupting normal EGFR signaling.
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